FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17686225
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC647650.36618894082824344No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT354880.2006533333144863No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA293480.16593704987921393No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA246240.13922699728178284No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT228300.12908350990672118No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT187190.10583943153499405No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT186140.10524574916354396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC345200.0113.8516541
CGGGTGC105550.0113.602261
CGGTTAA723850.0110.742931
CGGGTAC134450.0109.716811
GGGCGCG407150.0103.13422
CGGCTAA7200.0101.206241
CGGGTAT396600.097.483031
CGCGGTG419550.095.864545
GCGCGGT415050.095.7477344
GGCGCGG425150.095.549373
CGGAATA217600.094.908071
CGGGTGT337200.094.696921
CGGATGC12150.093.618061
CGGTAAT133250.093.587871
CGGGCGT524050.093.547011
GGGAGGC477300.092.680462
CGGGAGG1134900.090.918121
CGGGTTT681100.089.7033841
GCGGTGG605200.089.573036
CGGATAC14700.089.468531
Produced by FastQC (version 0.11.5)