FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16565776
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT567470.3425556400134832No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA421830.2546394445995165No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT337860.20395060273663002No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA320200.19329006984037453No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC303310.18309435066609617No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA279340.16862475986636546No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT222890.13454848115777976No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT201280.12150351423319981No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT198490.11981931905876307No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT194920.11766427362050531No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG184180.11118102768020043No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATTTCGTTATTGTATTTTAGTT170060.10265743059667111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC75150.0108.7400361
CGGTTAA570750.0108.4410251
CGGCTAA5600.0107.3448641
CGGGCGC184850.0101.681181
CGGGTAC79200.099.572331
GGGAGGC574050.099.47962
CGGGAGG1253650.097.89021
CGGTAAT128500.095.923481
CGGGTGT248700.093.644441
GCGGAGT382550.092.699427
CGGAATA238450.092.353281
CGGGTAA91650.090.4620741
CGGGAAG182850.089.122311
CGGGTAT272000.088.051551
CGGTATT294650.087.120621
CGGGGAC26250.086.8395161
CGGGTTT531150.086.327081
GGCGGAG678200.085.856026
GGAGGCG664550.085.679443
CGGTATA93000.085.501051
Produced by FastQC (version 0.11.5)