FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19783273
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT691990.34978539698663613No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA548290.2771482757175721No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC467110.23611360971463116No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT424560.21460554075152277No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT411120.20781192272886292No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA317790.1606357047188299No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA271410.13719165681027604No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT252690.12772911742157125No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA247210.12495910054923672No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA246020.12435758228681372No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA220210.11131120720014327No Hit
CGGTGGATTTTTCGGTTTAAGTTTTGGTAATACGGTGAAATTTCGTTTTA219690.11104835888379036No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT215250.10880403864416165No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT205390.10382003018408531No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT201230.10171724365326201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC105650.0111.715291
CGGTTAA760700.0109.266471
CGGGCGC277750.0105.1743551
CGGCTAA5500.0103.888611
CGGGTAC122100.0103.878861
GGGAGGC695900.097.086622
CGGGAGG1549600.095.382551
CGGGTGT356850.094.320551
CGGAATA301450.094.161071
CGGGTAT395150.093.553271
CGGTAAT126550.092.0894551
CGGGTAA116950.091.505851
GGGCGCG346700.090.330992
CGGGTTT733500.088.9181751
GCGGAGT457150.088.034257
CGGGAAG205250.085.487721
CGGGCGT510700.085.042861
CGGAATG1483350.084.98891
CGCGGTG354000.084.0720445
GGCGCGG360000.083.712293
Produced by FastQC (version 0.11.5)