FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17735019
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT346470.19535924940367982No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA313060.17652081455339855No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA258960.1460161954154095No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT242150.13653777309175705No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT242070.1364926645976528No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT186340.10506895989228994No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC184160.10383975342794953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC60350.0104.136031
CGGTTAA473000.0102.8964541
CGGCTAA5900.0100.8700941
CGGGTAC56250.095.221361
GGGAGGC408500.093.874162
CGGGAGG962400.092.609311
CGGGCGC135950.090.922561
CGGGTGT245100.089.3307341
CGGGTTT613550.088.0745241
CGGAATA200600.087.341631
CGGGTAA84350.087.276851
CGGTAAT91700.086.836271
CGGAGTG98150.086.101851
GCGGAGT270350.084.753237
CGGGTAT245950.084.666481
CGGATGA43150.083.9945141
CGGGATA75100.083.920951
CGGGAAG141800.083.520151
CGGAGTA95200.079.955441
CGGTATT232950.079.632161
Produced by FastQC (version 0.11.5)