FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16307592
Sequences flagged as poor quality0
Sequence length125
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC368030.2256801617308061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC59300.0202.807661
CGGGCGC196200.0201.222891
CGGCTAA3600.0196.156311
CGGTTAA453750.0191.23111
CGGGTAC79200.0180.93021
CGGGTAT258600.0152.079791
CGGGTGT211250.0150.733761
CGGATAC10400.0149.380581
CGGGCGT327100.0147.898451
CGGATGC6300.0146.578341
CGGAATA98450.0138.048931
CGGTAAT84000.0136.576541
CGGGTTT504150.0133.756061
CGGGAGG672100.0133.404161
CGGAAAT64500.0129.35791
CGGGAAA53450.0129.270921
CGGGTAA68250.0128.776721
CGGGCAT2650.0127.08881
CGGAGTA82150.0125.237341
CGGGGAC16700.0124.903961
Produced by FastQC (version 0.11.5)