FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18906384
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC463220.24500718910607125No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT227000.12006526472751215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA988550.0111.586871
CGGGTGC95600.0110.686091
CGGGCGC280300.0110.6200261
CGGGTAC121400.0106.036891
GGGCGCG334700.099.440682
CGCGGTG344050.093.179845
CGGGTAT391750.092.533441
GGCGCGG349450.092.166263
CGGGCGT492900.091.49871
GCGCGGT345250.091.2370154
CGGGTTT1060250.091.197731
CGGGTGT345750.090.626521
CGGAATA137500.088.6433941
CGGAAAT99800.087.183841
CGGAATT212450.087.120991
CGGTAAT119250.086.788131
CGGATAA81750.085.9041
CGGTGGT651750.084.162487
CGGAGTG107000.083.764561
CGGATAC18950.083.5394441
Produced by FastQC (version 0.11.5)