FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19907827
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT707480.35537781195305745No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC531430.26694525725987067No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA514300.25834060141270065No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT397150.19949439986594214No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA382860.19231631860172382No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA215890.1084447840540306No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT205870.10341158781417982No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT203640.10229142537756632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC100000.0113.052991
CGGTTAA716200.0110.870251
CGGGCGC291550.0109.502331
CGGCTAA7850.0109.3221
CGGGTAC106850.0107.980561
GGGAGGC608100.097.46172
GGGCGCG359750.096.5674742
CGGAATA284650.094.318821
CGGGAGG1408650.093.849491
CGGGTGT353050.093.617791
CGGGTAT393350.093.480441
CGGTAAT145050.093.471321
CGGGTTT715900.091.162491
CGGTACT1250.090.585421
CGGGTAA106050.090.531471
CGGGAAG206050.090.152741
GGCGCGG374500.089.7301943
CGCGGTG375250.088.964315
GCGCGGT374300.088.395474
CGGATAC13500.087.406981
Produced by FastQC (version 0.11.5)