FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30084332
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG478770.15914263943105003No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG403750.13420607112034263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC101950.034.2920151
TATCCGA54550.033.36717248
CTATCCG56300.032.39109847
TTACCGT25300.031.53401619
ATACGTC59200.030.38751238
TACCGTT26050.030.35752720
TACGTCC61650.029.46419339
GATACGT64350.028.17307337
GTTACCG29150.027.60922618
ACCGTTT28600.027.40612621
TGATACG67800.026.89439436
GTGTAGT79600.023.4747945
GAACCCC171400.023.4189471
AACCCCT160750.023.4011332
AACCCCC159400.023.2919832
GTAGTAT79350.023.2836977
TAGTATC81150.022.8103968
GCGTAAC19800.022.79833
TCTTATC84300.021.91650618
TTGGGGG209150.021.6516591
Produced by FastQC (version 0.11.5)