FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27687790
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG407400.14714067103224923No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307700.11113201884296292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG51700.034.32512347
TATCCGA55000.032.52164548
TACCGTT25150.032.1395720
TACGTCC57400.031.15774739
TTACCGT25500.031.14954419
TAACCCC91100.030.9232481
ATACGTC58550.030.6048838
GATACGT59800.030.25766437
TGATACG61800.029.22171436
GTTACCG28550.027.94439718
GTAGTAT66750.026.9451277
ACCGTTT30400.026.70425421
TAGTATC71850.025.3734388
GTGTAGT71600.025.266825
TCTTATC76200.023.32785218
TGTAGTA81350.022.1094866
TATATTA82700.021.84739761
GCGTAAC19550.021.8368153
TGCGTTA36700.021.83410815
CGTTACC36950.021.5916817
Produced by FastQC (version 0.11.5)