FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33297576
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG585310.17578156439976292No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG430360.12924664546151948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGT31500.029.66170719
TAACCCC164150.029.5371111
TACCGTT32900.027.33692220
ACCGTTT35250.026.01128221
TAGCGCG14900.025.6093981
TTGGGGG226150.024.2257331
GTTACCG38500.023.6323418
ATACGTC31000.023.37204638
TAGGGGG77800.023.308231
TATCCGA31500.023.22730448
CGTTACC40200.022.71984317
TTGCGAG33150.022.4934041
TCGCACA48850.022.1438471
TCGCAAC34750.021.86071
TCGCATT26400.021.481781
TGGGGGG404000.021.0997241
TCGCAAG53600.021.0958421
GCGTAAC20400.020.7567373
TTGCGGG52350.020.3958721
TACGTCC35750.020.36546139
Produced by FastQC (version 0.11.5)