FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33544164
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA666790.19877973408429558No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG543020.16188210861358773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG468720.13973220498206484No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA379510.11313741490173969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCG54050.033.529576
TGTACGA29600.032.3843575
CGGACTA20400.032.2406735
TATCGGA58200.031.441258
TTATCGG58250.031.413147
CGGAATT60200.030.57264311
CGCCCTA132200.030.14293961
ACCGTCC182850.028.53110910
TCTAGCG69100.028.41038733
TCGGAAT67600.027.38072610
CGGAACG75000.026.9751946
TGCGTAC66200.026.9505653
ATGTACG33050.026.886134
CTGTCCG174650.026.6042238
CACCGTC201400.026.2328249
CTAGCGG75200.026.19959334
AACGGCG78400.025.76285249
ATCGCTC68750.025.2447428
GTCGGTA19100.025.093044
TCGTTAT56750.025.0280064
Produced by FastQC (version 0.11.5)