FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33055435
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG613050.18546118058951577No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG512850.15514846499524207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGT42100.043.59418519
TACCGTT43850.041.4615920
GTTACCG47500.038.49260718
TGCGTTA48300.038.28405815
TAACCCC153950.037.4330061
CGTTACC49250.037.1249617
GCGTTAC49200.036.9525616
ACCGTTT53950.033.7641621
TAGCGCG14750.033.4550671
CTGCGTT57050.031.92860414
CGTTTGA59250.031.50104723
CTATCCG45600.030.4148647
TGTACCG60550.030.14040434
TACGGCC46750.030.0334517
CCGTTTG62700.029.76773822
GTCTACG47500.029.6347354
TCTCGTC45550.029.46155436
AACGCGC46100.028.81273325
TATCCGA48450.028.6968348
GCGTAAC22450.028.277173
Produced by FastQC (version 0.11.5)