FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33055435
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG448650.13572654542286314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG357080.108024595652727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC32500.040.446135
GCCGATC78200.034.63186669
ATTGCCG62000.033.6647562
GCGAACT51650.032.3294226
GGCCGAT85550.031.61615868
CCGCGAA40100.031.0617894
TACGAAC26650.028.9992085
CAATCGA47250.028.47441535
TTGCTCG925400.026.9203669
TGCCGAC78600.026.5110464
ATCGCAC1010050.025.53378120
AATCGCA1021900.025.30198319
CGACCTC82800.025.24945667
TCGCACT1023700.025.16635521
TCAATCG53600.025.10104634
AAATCGC1037300.025.02619418
CGCACTG1049500.024.48515922
ACCACGA1003250.024.31935354
TTGCCGA87400.024.03917563
GACTCTT1069850.023.81638136
Produced by FastQC (version 0.11.5)