FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28267602
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG584630.2068198073540161No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA502220.17766629090079875No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG484820.17151083420517949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC97150.030.90997969
GGCCGAT99650.030.13323268
ATCGCAC391400.029.34142520
AATCGCA398550.028.8750319
CCGATCC104150.028.59883770
AAATCGC407900.028.38546618
TCGCACT411350.027.92780121
TGCTCGC385850.027.79382570
TACGCTG65450.027.70166451
GACTACG67000.027.63717548
CTACGCT66050.027.45109650
ACTACGC67250.027.43013849
ACCACGA367150.027.27470854
ACGAACT381900.027.19292857
TTGCTCG404550.026.46436569
CGCACTG441550.025.97899622
CGAACTG405650.025.96231558
CAAATCG448050.025.8723917
CACGAAC398100.025.11819556
TCCTATC441400.025.0605544
Produced by FastQC (version 0.11.5)