FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35905853
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG675380.18809746700628446No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG533730.1486470743363206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC178700.035.8308371
TAGCGCG18500.034.6295741
TTGCGAG34550.027.6619241
TTGGGGG282450.026.610821
TTACCGT28050.026.07809819
TTGCGAC24200.025.460391
TACCGTT29100.025.25737820
GTTACCG29700.025.10058818
TAGCGCC34050.024.1612011
TAGCGAG29550.023.9310461
TCGCAAC36600.023.3386941
AACCCCC197750.023.0977272
TAGGGGG89500.022.8432331
TCGCATT32650.022.73111
TTACGCC22150.022.2850281
TAGCACG23350.022.1892491
CGTTACC33350.021.93372317
TGCGTTA33150.021.85513915
TAGCGGG30650.021.8157751
TGGCACC233950.021.4133361
Produced by FastQC (version 0.11.5)