FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37322536
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG654660.17540608708904454No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG456340.12226929059697338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC195250.041.755181
TTACCGT44650.041.5613519
TACCGTT46200.040.16784320
ACCGTTT51600.036.2320121
GTTACCG57800.032.28468318
TAGCGCG20150.032.018741
CGTTACC59150.031.54775817
CTGCGTT61750.030.3308214
CCGTTTG63800.029.62778922
TGCGTTA63750.029.32547615
TGTACCG64300.028.75516334
GCGTTAC67400.027.7885516
CGTTTGA68650.027.58491923
TTGGGGG292450.027.2519781
TATCCGA53100.026.76796548
ATACGTC53850.026.58300438
TTGCGAC26550.026.2497121
AACCCCC232300.025.9575062
CTATCCG54650.025.94535847
TACGTCC54850.025.84663239
Produced by FastQC (version 0.11.5)