FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27863196
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG488730.17540342464661987No Hit
TCCTTGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC295170.10593544258167654No Hit
TCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC291060.1044603784863732No Hit
TCCTGGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC290240.10416608346005964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGC985300.033.91836570
GTTATAC89950.033.5028718
CGCGAAC28000.033.231555
AACGCAC81700.032.60598436
ATCGCAC1136900.031.700920
AAATCGC1154600.031.49055918
AATCGCA1145700.031.47726419
GCGAACT39550.031.310956
TCGCACT1153650.031.24237321
ACCACGA1023550.030.5477554
TTGCTCG1113900.030.24302169
CGCACTG1194150.030.13424722
ACGAACT1096700.029.96161757
ATTGCCG44350.029.91052462
GGACTCT1158600.029.8538635
GACTCTT1150800.029.78559536
GAACGCA89550.029.6694435
CCACGAA1060150.029.64756455
CACGAAC1064200.029.6260356
TGAACGC90100.029.60599934
Produced by FastQC (version 0.11.5)