FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29605357
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG685130.23142095533588736No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG502160.16961795123767634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC115050.031.822481
GCGTAAC24300.029.812023
TTGGGGG216700.028.2823931
TAGGGGG57750.024.0617641
TTGCGAG29650.023.9640521
TGGGGGG399950.022.8327031
TACCGTT23750.022.69287720
TTGCGAA22500.022.556641
CGCGTAA5650.022.2988322
ACGTAAC20100.022.1123543
TCGCTAG14100.022.093261
TTGCGAC23850.022.0136381
TCGCACA41050.021.8280891
TTACCGT24950.021.60118119
TAGCGCG13350.020.974791
TTGCGCT26200.020.5735071
ACCGTTT26750.020.27891721
TTACGCC18000.020.028741
GTTACCG26200.019.90271418
TTGCACC83350.019.820991
Produced by FastQC (version 0.11.5)