FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32152833
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG672780.20924439224375657No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG502170.15618219396094896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC163800.043.720061
GCGTAAC26450.032.945483
TTGGGGG248750.030.2808571
TTGCGAG31250.028.8973941
TTGCACC119700.028.773321
TTACCCC95050.027.9866221
TAACCCG23050.027.4850581
TAGCGCG16500.026.9406931
TTGGGGC182250.026.3112831
TAGGGGG72450.026.0882151
TTACCGT23200.025.94549219
TTGCAAC104950.025.3799781
TTGCGAC24300.025.3510171
TACCGTT24050.025.31976720
TCGCACA46250.024.595781
TGGGGGG445000.024.1393681
GTTACCG25250.023.97773718
TTGGGAC154850.023.5303571
TTGCATC89250.023.177591
TTGGGCG54300.023.076621
Produced by FastQC (version 0.11.5)