FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27758204
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG431770.15554680699082693No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG282360.1017212785092292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGT27950.039.68711519
TACCGTT31250.035.8320820
GTTACCG32050.034.93767518
TATCCGA60550.033.24012448
ACCGTTT34150.032.9941721
CTATCCG60750.032.72606747
ATACGTC64850.031.67933338
GATACGT67250.030.60075837
TACGTCC67350.030.45221939
CGTTACC38150.029.99337217
TGATACG69800.029.33234836
TGCGTTA39400.028.86423115
TAACCCC83700.027.6798211
GCGTTAC41550.027.03373716
GTGTAGT79550.026.6136025
TAGTATC80300.026.4512848
GTAGTAT79250.026.448467
TGTACCG43700.026.3478534
TCAAACG119750.024.03857869
CGGTACA122400.023.70875458
Produced by FastQC (version 0.11.5)