FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33657989
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG535070.1589726587646101No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG457920.13605090904272385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC87150.030.64028269
CGCGAAC23500.030.3902745
ATTGCCG43700.029.44715562
ACTACGC82650.028.71891249
CTACGCT82200.028.7079550
TACGCTG82250.028.64889551
GCGAACT35150.028.5620546
GACTACG84200.028.31299648
GGCCGAT99150.026.89661268
CGATTAC61650.026.74864210
TATCGAT63150.026.3872177
TTGCTCG520250.025.67689369
TTATCGA64450.025.5339056
CGGATAG78250.024.82071335
ATCGCAC549850.024.72554820
TCGGATA78450.024.58157234
AATCGCA563250.024.19265219
ATCGATT69400.024.1595158
TCGCACT568200.024.00585621
ACCACGA544550.023.68223454
Produced by FastQC (version 0.11.5)