FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34746507
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG618750.1780754537427316No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG448620.1291122586796998No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351800.1012475872754634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG61750.039.6067624
TCTACGG62150.039.185345
TACCGTT34550.035.84095820
TACGGCC68300.035.5017437
TAACCCC196600.035.20471
TTACCGT35600.034.87983719
TCTCGTC68450.034.7227436
TGCGTTA39350.030.94216315
GTTACCG40750.030.3023818
GCCCGAT78050.030.27390130
TAGCGCG15800.030.1352791
CCCGATC79300.030.01513931
ATACGTC58700.029.61620338
AACGCGC80750.029.431525
TATCCGA58200.029.22433548
TACGTCC58850.029.13042839
TCGTCTG82550.028.83161538
CTACGGC86200.028.812876
GCGTTAC42400.028.63506116
ACCGTTT42550.028.5350621
Produced by FastQC (version 0.11.5)