FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34746507
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG611970.17612417846778095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC30100.033.109355
TTGCTCG819350.030.79652869
GCCGATC71000.028.38892469
GCGAACT45500.027.4366
ATCGCAC957700.027.4351220
ACCACGA917750.027.06708754
TCGCACT977300.026.89545221
AATCGCA979100.026.88774319
TACGAAC22000.026.8035745
AAATCGC987900.026.74883518
ACGAACT964700.026.3226957
CGCACTG1002750.026.1992722
TCCTATC998800.025.79835544
GGCCGAT77900.025.78605768
CACGAAC971750.025.61971356
CCACGAA972400.025.57752855
GACTCTT1015450.025.38426236
GGACTCT1020150.025.25749235
CGAACTG1009150.025.22552958
CCTGGAC1033350.025.06478132
Produced by FastQC (version 0.11.5)