FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23032821
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG548270.23803857981616752No Hit
GCTGCTGTCTTCCTAGATTCACTGAATCCACTTCTGTGTAGCACCTGGGT404560.1756450067492818No Hit
TCCTGGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC356940.15497016192675658No Hit
TCCTTGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC344340.14949970739580704No Hit
TCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC338710.14705536937920022No Hit
TCTGTGTAGCACCTGGGTCAGCTGTCAATTAATGCTAGTCCTCAGGATTT240780.10453778110809787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC24100.044.863855
CGTACGG30650.040.76355443
GCGTACG31000.040.64341742
CCGCGAA25650.040.1054764
ATTGCCG38750.036.40531562
GCGAACT37850.034.8539666
GGCGTAC36400.034.71042641
TGCTCGC1094950.034.6013770
TACGAAC17400.034.3876045
GCGGTTA38700.033.36964434
CGTTAAT32150.033.09380741
ACCGCGA21650.032.487683
TCGTTAA33350.032.11305240
ATCGCAC1228000.031.877920
TCGCACT1235800.031.68519421
AATCGCA1237050.031.6786419
AAATCGC1242250.031.65812318
CGCACTG1253800.031.16317422
ACCACGA1181800.030.96586854
GACTCTT1244150.030.70371636
Produced by FastQC (version 0.11.5)