FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34139130
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG691640.20259450079717906No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG474780.13907208531676116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC140300.043.3660281
GCGTAAC31350.032.5946543
TTACCGT29350.032.31041319
TACCGTT30800.030.33640920
TCGCAAC35400.030.2604731
ACCGTTT32550.029.3509121
TCGCACA52150.029.2677761
TTGGGGG243650.028.5488761
CGCAACT33050.027.8476472
TAGCGCG16000.027.7869761
TAGGGGG78300.026.4230691
TCGCAAT24500.025.2909261
AACCCCC170700.025.2364792
CGCGTAA6700.025.0709422
TGGGGGG436100.024.8767151
AACCCCT174350.024.2465082
TGGGTAG114400.024.1745621
TCGCATG27450.023.9757981
TCGCTAG15050.023.9584241
TTGCGAG31600.023.8181971
Produced by FastQC (version 0.11.5)