FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27891706
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG542180.19438753585026314No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG413190.1481408128997201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC131400.035.5154081
TACCGTT24300.030.53472720
TAGCGCG12000.030.0495531
GTTACCG25300.029.46568518
TTACCGT25800.029.1664819
TTGGGGG201250.028.4248521
CGTTACC27050.027.94751217
GCGTTAC28750.026.1733216
TAGCGGA18250.025.5135481
TGCGTTA30000.025.19947615
ACCGTTT30450.024.71248421
TTGCGAC14450.024.7123661
TTGCGAG23250.024.5440751
TCGCACA36900.024.477971
TTGCGGG37050.024.1898841
TAGGGGG59150.024.0299741
GCGTAAC18650.023.2715473
TGGCACC169300.022.870741
TCGCATT25150.022.6898521
TTGGGAC114650.022.6574971
Produced by FastQC (version 0.11.5)