FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27891706
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG631540.22642573387228448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC20350.033.536345
TGCTCGC749100.031.89226570
ATTGCCG33700.030.93523462
ATCGCAC842150.030.00773620
TCGCACT852650.029.60910421
AATCGCA857550.029.53424519
AAATCGC867450.029.35067618
ACCACGA785650.029.12505154
CGCACTG878850.028.68913322
TTGCTCG845450.028.60690169
ACGAACT840800.028.50801557
GCGAACT30400.028.2064766
CACGAAC817750.028.03273656
CCACGAA820800.027.89255355
GGACTCT886950.027.62804635
GACTCTT883600.027.62728136
CGAACTG872450.027.57280758
TCCTATC895600.027.55753744
CGTACGG19050.027.20961843
CCTGGAC906250.027.07973732
Produced by FastQC (version 0.11.5)