FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24156380
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG438020.18132683787885434No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325430.13471803308277153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323790.13403912341170324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC92300.034.3532261
TATCCGA36400.029.3830548
CTATCCG36850.029.11683847
TACGTCC39600.027.87633539
ATACGTC39550.027.56268138
GATACGT42500.025.73051637
TTGGGGG183300.025.1288411
TGATACG46200.024.04318836
TAGGGGG48700.022.5295161
GTAGTAT51950.021.7140717
GTGTAGT53500.020.8931755
TGGGGGG322400.020.461931
TAGTATC55200.020.4356178
TTGCGAG26700.020.0296861
AACCCCC129150.020.0076942
TAGGGAG87300.019.9191131
TCGCATA8950.019.6607841
TTGGGGC130400.019.579781
TCGCATG18850.019.4020771
TAGCACG16800.019.0997351
Produced by FastQC (version 0.11.5)