FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25423282
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG506610.19927010210562113No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG352050.1384754336595881No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288230.1133724591498454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC121850.035.9033851
CTATCCG42400.030.91959447
TATCCGA43450.029.93537548
ATACGTC45000.029.2069238
TTACCGT19950.028.87682519
GATACGT45950.028.7531737
TACCGTT20350.028.64836720
TACGTCC47500.027.59709239
TTGGGGG210150.027.0563661
TGATACG50100.026.23371136
ACCGTTT24550.024.30923821
GTTACCG26100.022.86549618
TTGGGGC158900.022.6899761
TGGGGGG374400.022.4482821
TAGTATC60950.022.1863828
TTGCAAC80000.021.9948941
AACCCCC151000.021.9559942
GTGTAGT61250.021.8540675
GTAGTAT61350.021.760637
AACCCCT164150.021.5631962
Produced by FastQC (version 0.11.5)