FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25423282
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398800.15686409016743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG364100.030.44513769
ATCGCAC411200.027.89065720
AATCGCA419650.027.4267719
AAATCGC423150.027.370618
TCGCACT425000.027.04290821
ACCACGA396100.026.6198954
ACGAACT415500.026.4748357
CGCACTG442900.025.87996522
CGAACTG428250.025.81551758
CACGAAC411650.025.76590756
CCACGAA415350.025.469855
CAAATCG470500.024.60084517
TCCTATC455700.024.57652944
GCCGATC39750.023.62272869
GACTCTT474900.023.62072636
GGACTCT478250.023.51321835
CACCACG458600.023.47257253
CCTATCT483550.023.1608245
TTCCTAT488600.023.06298343
TGGACTC500650.022.78458234
Produced by FastQC (version 0.11.5)