FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25616735
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG357240.13945571127624187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG451100.032.59330769
ATCGCAC524550.029.19506320
AATCGCA529900.028.9445119
AAATCGC534350.028.79971918
TCGCACT533050.028.71139521
ACCACGA505900.028.08171754
ACGAACT528450.027.84554957
CGCACTG554200.027.57871422
CACGAAC520550.027.33236356
CGAACTG541800.027.12763258
CCACGAA526850.026.9923255
TCCTATC566200.026.61081744
GACTCTT589350.025.69037436
GGACTCT589350.025.6201235
CAAATCG602900.025.58765417
CGCGAAC14700.025.5639655
CCTATCT596350.025.1955245
CACCACG574800.025.17671253
TTCCTAT603350.025.00129543
CCTGGAC617100.024.50433732
Produced by FastQC (version 0.11.5)