FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36544422
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG815200.22307097920443233No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571750.15645342536817247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG88400.054.3224
TCTACGG91150.052.9519655
TAGCGCG50400.049.7340551
TACGGCC98700.049.0767257
AAACGCG32350.047.5074351
CTACGGC105500.045.815976
TTGCGAC24600.045.6815951
CGTTCGA26750.045.66197636
TATCGCG9800.045.0107041
CTCGCGT31900.044.54416726
CGGCCAT108300.044.5326589
TACCGTT34100.044.1341620
TAGCGGG40800.041.87271
GAACGCG110550.041.85320724
ACGGCCA118750.041.0852368
ATACGCG8700.040.6418531
GTTACCG37000.040.48575618
GCGTTAC37250.040.12019316
TGCGTTA37350.040.10648315
TTACGCC27250.039.6975141
Produced by FastQC (version 0.11.5)