FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27828253
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590660.21225191534660834No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG472810.16990286813908154No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320290.11509525948323095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC149100.042.846261
TTACCGT29950.038.56347319
TACCGTT30200.037.66491320
GTTACCG33300.034.6841518
ACCGTTT33550.034.42588421
TAGCGCG12350.030.894931
TTGGGGG234200.030.5058981
CGTTACC38650.030.06416717
TAGGGGG66900.027.836411
CCGTTTG43800.026.9287622
TGTACCG42100.026.85366834
CGTTTGA44350.026.83145923
TTGCGAC18550.026.7961311
TGCGTTA43600.026.6508715
CTGCGTT43650.026.61991114
GCGTAAC19700.026.1164443
GCGTTAC44900.025.8792416
AACCCCT183500.025.6539232
AACCCCC164950.025.5683272
TCGCACA42900.025.3764461
Produced by FastQC (version 0.11.5)