FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27828253
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG617180.22178179851965554No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA279020.10026500765247463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC24150.030.5672555
TGCTCGC716750.029.68178270
ATCGCAC774850.029.20481120
AATCGCA783700.028.91556419
AAATCGC790800.028.8286818
TCGCACT790400.028.62232221
ACCACGA722650.028.20513354
CGCACTG818500.027.56337522
ATTGCCG36100.027.54346762
ACGAACT783100.027.3287457
TTGCTCG798000.027.03932269
TCCTATC814300.027.0263144
CACGAAC756700.026.97764656
CCACGAA759200.026.8470255
GACTCTT820750.026.5866436
GGACTCT831400.026.46013835
TGGACTC843950.026.32899934
CGAACTG819300.026.18515258
TTCCTAT846500.026.00554543
CCTGGAC849200.026.00446532
Produced by FastQC (version 0.11.5)