FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32257495
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564750.17507559095955838No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG413930.1283205654995839No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330300.10239480778033137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC166950.040.4649161
TTACCGT34550.038.5359819
TACCGTT36000.036.7931220
GTTACCG39400.034.22990418
ACCGTTT39300.033.70377721
CGTTACC41200.032.3995417
TGCGTTA44000.030.18087615
CTATCCG52350.028.99602947
CTGCGTT46950.028.35777514
ATACGTC54750.028.22481738
TATCCGA54300.028.20917548
GCGTAAC26750.027.209983
TAGCGCG12600.026.835391
TACGTCC57800.026.73540339
GATACGT58400.026.69772737
TAGCGGA24750.026.486881
GCGTTAC51250.026.24797816
TTGGGGG246850.026.0534441
TGTACCG52000.025.54023634
CCGTTTG53000.025.38204622
Produced by FastQC (version 0.11.5)