FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34036170
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG844760.24819478807398132No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA631850.18564074630018595No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA394980.11604713456302515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCG81850.036.1666036
TTATCGG83800.036.0777787
TATCGGA84050.035.9318
TCTAGCG109900.033.27817533
CGGAATT91650.032.95450211
CTAGCGG116400.031.63032734
TCGGAAT96700.031.26910210
ATCGCTC99350.030.15599328
TAGCGGC124800.029.6697135
CAAATCG102000.029.40692925
AATACGA123900.029.31951144
TCGTTAT77550.028.920564
ATACGAA126500.028.71571545
CGCAATA128450.028.5819141
CGAATGC126450.028.3116948
CAATACG131050.027.77323243
CGTTATC91700.027.2816775
AATCGCT109900.027.26128627
GCGCAAT136450.026.93201340
TTCGTTA82650.026.3778173
Produced by FastQC (version 0.11.5)