FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30387993
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG456800.15032253034940476No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG433170.14254643273084866No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATC325790.10721010762375785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC84000.035.52627669
ATTGCCG49200.032.9573362
GGCCGAT95100.031.48840168
CGCGAAC22150.030.373675
TTGCTCG695400.030.14861569
ATCGCAC794050.027.22717320
CGATTAC35300.026.97393810
AATCGCA805950.026.91513319
AAATCGC809600.026.87892318
TCGCACT808150.026.7606721
ACCACGA775050.026.34706154
TACGAAC16450.026.2168525
CGCACTG826750.026.16278822
CGTACGG27650.025.82720243
TGCCGAC62700.025.80618964
ACGAACT820700.025.59201657
TCCTATC840050.025.39644444
CACGAAC812500.025.18790656
CCACGAA814700.025.11561255
GGACTCT853850.025.03348235
Produced by FastQC (version 0.11.5)