FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30219511
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG418890.13861574398076792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA72050.034.15284748
TTACCGT30750.033.9115319
CTATCCG74200.033.1616547
TACCGTT32050.033.0819820
ATACGTC76150.032.8612638
TACGTCC78800.031.66769239
GATACGT80650.031.07120937
GTTACCG34250.030.65045418
ACCGTTT35700.029.50361321
TGATACG85650.029.42095836
TAACCCC91150.028.9101091
GTAGTAT94450.026.638057
TAGTATC97400.026.1905618
CGTTACC40350.025.75659217
GTGTAGT98500.025.542955
TCTTATC102800.024.50843818
CTCTATC107300.023.32231345
TATATTA109700.023.30009561
TGTAGTA108950.023.1893396
AGTGTAG111850.022.8383774
Produced by FastQC (version 0.11.5)