FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30219511
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG642790.21270694949365657No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG397010.1313753885693253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC92450.032.84289669
GGCCGAT99700.030.77121768
TGCTCGC543350.030.49885270
CCGATCC102250.029.62587770
ATCGCAC598450.029.3185220
AATCGCA607500.028.99775919
AAATCGC610950.028.98949618
TCGCACT615900.028.46991721
ACCACGA566200.028.1294454
ACGAACT586650.028.04675557
TTGCTCG600150.028.03901969
CGCACTG632200.027.59675222
CGGATAG62650.027.4830335
CGAACTG607100.027.25172658
TCGGATA62500.027.21167234
CACGAAC586550.027.14287256
CCACGAA593650.026.74652555
TCCTATC644800.026.3134144
CAAATCG682850.025.96312517
GGACTCT667950.025.52606435
Produced by FastQC (version 0.11.5)