FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34763620
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG601930.1731494015870614No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG532990.15331832530674308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT84600.058.11335426
CGATCCA92150.053.7646455
TCGCGTC91400.053.75292227
TCTACGG99750.053.542145
GTCTACG103050.051.9951744
TACCGTT52050.051.7651420
AAACGCG32150.049.9111671
TAACCCC232950.049.7295341
GCGATCC100600.049.283144
TACGGCC108700.049.0015537
TAGCGCG60950.048.861061
TGCGTTA56000.047.98994415
GAGTCGC103950.047.557236
GCGTTAC57150.047.38652416
AGAGTCG105150.046.8177535
CGTTACC57950.046.67262617
GTTACCG58300.046.39222318
TTACCGT58700.046.2525419
ACCGTTT58900.045.8626521
CGGCCAT116700.045.760789
Produced by FastQC (version 0.11.5)