FastQCFastQC Report
Wed 1 May 2024
EGAF00005420150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34763620
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA1035300.2978113326517779No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG646060.18584370672559417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG604470.17388005046655094No Hit
GGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCG603950.17373046880618304No Hit
GCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGG569820.16391273405934134No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG548530.15778851569543104No Hit
ACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGC485920.13977830847305314No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG479230.1378538828810118No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA470150.13524195696535632No Hit
GGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCG425980.1225361455452568No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA392240.11283059704369107No Hit
GTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGT384630.11064152697561416No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA383390.11028483224704447No Hit
TTGACATTCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGC379110.1090536601194007No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACGTCGCCGCC370790.1066603535535137No Hit
TCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAG369130.10618284286849299No Hit
GTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGA364920.10497180673359104No Hit
GGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCC348150.10014779818672509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)