FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38208573
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG756610.19802100434371103No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATC634830.1661485761323774No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG400280.10476182923659567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC158100.039.90549569
GGCCGAT180450.035.03960868
TTGCTCG1124200.034.2862769
ATCGCAC1321650.030.17167520
ATTGCCG68450.030.04796862
AAATCGC1343850.029.90807318
AATCGCA1339800.029.82668919
TCGCACT1344200.029.66548521
ACCACGA1287950.029.41987854
CGCACTG1369300.029.09414322
CGGATAG131000.029.07704435
TCGGATA132550.028.86687334
ACGAACT1338400.028.81096657
TCCTATC1376350.028.46549244
CACGAAC1337500.028.35789956
CCACGAA1337750.028.31132955
GACTCTT1387700.028.25679636
GGACGTA136600.028.18807243
GGACTCT1393700.028.1848835
CGAACTG1378000.027.99888658
Produced by FastQC (version 0.11.5)