FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32979124
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG712530.2160548594316817No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG655540.19877423063147465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC261050.044.603351
TACCGTT36700.041.7514720
TTACCCG38750.039.9981461
TTACCGT41150.037.66098819
TAGCGCG25750.037.500731
TTGCGAC31250.037.1702841
TTGGGGG360150.036.6142851
ACCGTTT42200.036.4758921
TTGCGAG43950.036.0617831
TTGGGCG84100.034.5294721
TGCGTTA45100.033.81913415
TTGCACC126400.033.7968671
GTTACCG46300.033.47196618
TTACCCC121550.032.526051
GTCTACG29500.032.2561574
CGTTACC48550.031.99269717
CGTTTGA47800.031.83696623
TTACGCC26700.031.4490721
TGTACCG49150.030.76944734
TTACGGC24050.030.4045831
Produced by FastQC (version 0.11.5)