FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32979124
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA472260.143199679894469No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG369380.1120041878613877No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG347210.10528175339041751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC64150.030.77502410
TTATCGA66850.029.2670046
GCCGATC89800.027.83643769
CGCCCTA75600.027.40756261
TATCGAT73500.027.0006857
GGCCGAT99400.025.28743668
ATCGATT78900.025.2418338
CCGATCC103650.024.05072670
TCTAGCG33100.023.36952633
ATCGCAC212550.023.00538420
TTTATCG84900.022.919965
GTTATCG34150.022.5475736
AATCGCA220500.022.22406619
ACGAACT212300.021.95598257
TCGCACT229850.021.50220921
ACCACGA209700.021.34274754
CGGTAGG70700.021.2374260
CTCGGTA70600.021.16693958
CGTGATA50500.021.14261467
TCGATTA94150.021.042969
Produced by FastQC (version 0.11.5)