FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29312319
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG616490.21031771658871481No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG488190.166547723501508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316310.10791026121133576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC148650.043.047021
TACCGTT29400.041.78500420
TTACCGT30050.040.64795319
GTTACCG35450.034.85119618
ACCGTTT37000.033.01278721
TAGCGCG14550.030.79491
TTGGGGG245450.030.3201961
CGTTACC40850.030.15845317
TGCGTTA41600.029.10988415
GCGTTAC41850.029.01967216
TTGCGAC19350.028.7639141
TCGCAAC32150.028.417861
TAGGGGG77500.027.4620951
TCGCACA46900.027.1681691
CGTTTGA45100.026.92849723
TGTACCG45750.026.5469534
CCGTTTG46300.026.53271322
CTGCGTT46950.026.31424514
AACCCCT178350.026.1399752
TAACCCG29750.024.944841
Produced by FastQC (version 0.11.5)