FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29312319
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG732230.24980282181017474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAC388050.027.97208220
AATCGCA396450.027.40640319
AAATCGC402750.027.08182118
TCGCACT404700.026.9605321
TGCTCGC391950.026.31280970
ACCACGA374950.026.11602454
ACGAACT401850.025.83104957
CGCACTG422450.025.72890522
TTGCTCG416350.024.97113469
CGAACTG424200.024.46999258
CAAATCG448650.024.34244517
CACGAAC406700.024.22334756
CCACGAA409650.024.05749755
TCCTATC451550.023.40317344
GGACTCT466450.022.83117935
CGCGAAC18100.022.6148765
CACCACG444300.022.42576653
GACTCTT474300.022.3131536
TGGACTC481050.022.21876734
CCTATCT477500.022.12375645
Produced by FastQC (version 0.11.5)