FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30782278
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG721900.23451805613606633No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG531500.17266428430020678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC154650.045.6956941
TTGGGGG256400.030.9745731
TAGCGCG14250.029.2294731
TTACCCC90200.028.831761
TTGCAAC99550.028.2685591
TTACCGT24600.026.46077319
TTGCACC105200.025.4194721
TACCGTT25350.025.26391420
TTGCGAC23000.025.2620811
TTGGGGC185200.025.1550961
TGGGGGG455250.024.6645571
TTGCATC90500.024.5205141
TAACCCG23550.024.3748421
TTGGGCC140300.024.2990991
CTATCCG35900.024.27519447
TTGGGAC153550.024.02591
TAGGGGG74100.023.9957541
TTGCGAG44350.023.8342881
ACCGTTT27350.023.6726121
ATACGTC37700.023.67241538
Produced by FastQC (version 0.11.5)