FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34729861
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG783790.22568187071062568No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG530800.15283677639827006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC186850.038.237811
GCGTAAC35400.035.7851873
TTACCGT27250.029.66331719
TTGGGGG265250.028.5195371
TCGCACA59400.028.2877161
TAGCGCG14900.028.1927911
CTATCCG53800.028.04634347
ATACGTC55100.027.82374438
TCGCAAC35100.027.7255861
TACCGTT28850.027.6560920
TATCCGA54550.027.33925648
TAGGGGG75600.026.7638841
ACGTAAC24750.026.722963
TACGTCC58350.026.09536439
GATACGT59650.025.75951837
TGGGTAG112750.025.489991
TCGCAAG60800.025.4484751
TTGCGAG35500.024.8493631
TGGGGGG490650.024.7428861
TCGCATA15450.024.6968231
Produced by FastQC (version 0.11.5)