FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34899200
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG772070.2212285668439391No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA372590.10676175958188154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC20700.031.1100235
TGCTCGC668850.030.80845870
ATCGCAC732650.029.36790520
AATCGCA743450.028.96485519
ACCACGA696500.028.6413154
TCGCACT754400.028.55802221
AAATCGC761450.028.3769418
TTGCTCG742100.027.943669
ACGAACT746550.027.921557
CGCACTG775750.027.76607322
GCCGATC62700.027.66798269
CCACGAA735250.027.1775555
CACGAAC741200.027.06135256
CGAACTG772250.027.01370258
GGCCGAT65300.026.5664668
TCCTATC799250.026.36573444
ATTGCCG40450.026.29158462
GGACTCT808050.026.2521635
TCTAGCG35500.026.13716533
GACTCTT807100.026.12021636
Produced by FastQC (version 0.11.5)