FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27296374
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG431640.15813089313620923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG446450.031.11708869
ATCGCAC513250.028.23861520
AATCGCA519150.027.95642519
AAATCGC524150.027.91290918
TCGCACT523200.027.60340521
ACCACGA484950.027.07363954
ACGAACT509250.026.92903957
CACGAAC502050.026.40654656
CGCACTG547900.026.34031722
ATTGCCG30050.026.30603862
CGAACTG528750.026.25583558
CCACGAA507450.026.1595555
TCCTATC549850.025.61314844
CAAATCG582650.025.11581817
GGACTCT581400.024.26119835
GACTCTT580850.024.15960136
CCTATCT580350.024.15353245
CACCACG555150.024.13385853
TTCCTAT592150.023.85944443
TGGACTC602650.023.60559834
Produced by FastQC (version 0.11.5)